Galazzo, Laura, and Enrica Bordignon. “Electron Paramagnetic Resonance Spectroscopy in Structural-Dynamic Studies of Large Protein Complexes.” Progress in Nuclear Magnetic Resonance Spectroscopy 134–135 (April 2023): 1–19.
Macromolecular protein assemblies are of fundamental importance for many processes inside the cell, as they perform complex functions and constitute central hubs where reactions occur. Generally, these assemblies undergo large conformational changes and cycle through different states that ultimately are connected to speciﬁc functions further regulated by additional small ligands or proteins. Unveiling the 3D structural details of these assemblies at atomic resolution, identifying the ﬂexible parts of the complexes, and monitoring with high temporal resolution the dynamic interplay between different protein regions under physiological conditions is key to fully understanding their properties and to fostering biomedical applications.