Using [1- C]lactic acid for hyperpolarized C MR cardiac studies

Chen, A.P., et al., Using [1- C]lactic acid for hyperpolarized C MR cardiac studies. Magn Reson Med, 2014: p. n/a-n/a.

http://www.ncbi.nlm.nih.gov/pubmed/25046652

PURPOSE: Hyperpolarized [1-13 C]lactate in solution may be a clinically relevant and safe substrate for real time MR investigations of key metabolic pathways. The potential of using hyperpolarized [1-13 C]lactate for magnetic resonance studies of cardiac metabolism in vivo was explored. METHODS: Neat [1-13 C]lactic acid was hyperpolarized using the dynamic nuclear polarization process. Cardiac MR spectroscopy experiments were performed in vivo using hyperpolarized [1-13 C]lactate and [1-13 C]pyruvate in solutions. RESULTS: A high degree of polarization was achieved for [1-13 C]lactate in solution (16.7%). 13 C-bicarbonate was observed in rat hearts in vivo after either hyperpolarized [1-13 C]lactate or hyperpolarized [1-13 C]pyruvate was infused, but lower 13 C-bicarbonate to substrate ratio was observed with hyperpolarized [1-13 C]lactate infusions. The response of 13 C-bicarbonate signal as a function of hyperpolarized [1-13 C]lactate doses was also investigated and a saturation of 13 C-bicarbonate signal was observed at the highest dose of [1-13 C]lactate used (0.69 mmol/kg). CONCLUSION: This study demonstrated that the use of neat [1-13 C]lactic acid as the DNP sample is a potential alternative to [1-13 C]pyruvic acid for cardiac hyperpolarized 13 C MR studies. Hyperpolarized [1-13 C]lactate may enable noninvasive assessment of cardiac PDH flux in cardiac patients in the near future. Magn Reson Med, 2014. (c) 2014 Wiley Periodicals, Inc.

Might this article interest your colleagues? Share it!

Have a question?

If you have questions about our instrumentation or how we can help you, please contact us.